Temperature-dependent Chaperone Activity and Structural Properties of Human αA- and αB-crystallins
نویسندگان
چکیده
منابع مشابه
Temperature-dependent Chaperone Activity and Structural Properties of Human aA- and aB-crystallins*
The chaperone activity and biophysical properties of recombinant human aAand aB-crystallins were studied by light scattering and spectroscopic methods. While the chaperone function of aA-crystallin markedly improves with an increase in temperature, the activity of aB homopolymer appears to change very little upon heating. Compared with aB-crystallin, the aA-homopolymer is markedly less active a...
متن کاملStructural and functional properties of NH2-terminal domain, core domain, and COOH-terminal extension of αA- and αB-crystallins
PURPOSE The purpose of the present study was to determine the biophysical and chaperone properties of the NH(2)-terminal domain, core domain and COOH-terminal extension of human αA- and αB-crystallins and correlate these properties to those of wild type (WT) αA- and αB-crystallins. METHODS WT αA- and αB-crystallins cloned into pET 100D TOPO vector, were used as templates to generate different...
متن کاملMultiple Aggregates and Aggresomes of C-Terminal Truncated Human αA-Crystallins in Mammalian Cells and Protection by αB-Crystallin
BACKGROUND Cleavage of 11 (αA162), 5 (αA168) and 1 (αA172) residues from the C-terminus of αA-crystallin creates structurally and functionally different proteins. The formation of these post-translationally modified αA-crystallins is enhanced in diabetes. In the present study, the fate of the truncated αA-crystallins expressed in living mammalian cells in the presence and absence of native αA- ...
متن کاملOligomerization with wt αA- and αB-crystallins reduces proteasome-mediated degradation of C-terminally truncated αA-crystallin.
PURPOSE We previously demonstrated that the ubiquitin-proteasome pathway (UPP) is a general protein quality control system that selectively degrades damaged or abnormal lens proteins, including C-terminally truncated αA-crystallin. The objective of this work was to determine the effects of wt αA- and αB-crystallins on the degradation of C-terminally truncated αA-crystallin (αA(1-162)) and vice ...
متن کاملInteraction of βA3-Crystallin with Deamidated Mutants of αA- and αB-Crystallins
Interaction among crystallins is required for the maintenance of lens transparency. Deamidation is one of the most common post-translational modifications in crystallins, which results in incorrect interaction and leads to aggregate formation. Various studies have established interaction among the α- and β-crystallins. Here, we investigated the effects of the deamidation of αA- and αB-crystalli...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.7.4565